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KMID : 1234420080360030189
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 3 p.189 ~ p.194
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Molecular Cloning of a Putative Gene Encoding Phospholipase B (plbA) from Aspergillus nidulans
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Hong Sa-Hyun
Eom Chi-Yong
Song Seung-Eun
Cho Eun-Min
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Abstract
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The phospholipase B (PLB) families are enzymes sharing phospholipase (PL), lysophospholipase (LPL) and lysophospholipase-transacylase (LPTA) activities. In this study, we report the putative gene encoding phospholipase B (plbA) containing lipase motifs was cloned for the first time from the filamentous fungus, Aspergillus nidulans. plbA was isolated from A. nidulans genomic DNA library using a PCR-amplified probe, which is designed on the basis of sequence information derived from the conserved lipase regions of various PLBs. The deduced product of plbA is of 626 amino acids. From the assigned sequence, PlbA showed 72% identity with Penicillium notatum PLB but have low similarity with phospholipase A of other organisms.
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KEYWORD
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Aspergillus nidulans, fungi, Phospholipase B, PLB, plbA
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